Effect Of IGF-1 On Expression Of Gh Receptor, IGF-1, IGF-1 Receptor, KAP3.2 And KAP6-1 MRNA In The Skin Of Sheep

Abstract

Thirty-six healthy and similar Chinese Merino sheep were selected and divided into six groups at random. The treatment group was injected intradermally with 0.5 mL IGF-1 (10ng/mL). Treatment skin tissue of sheep were sampled respectively for 0,3,6,9,12 and 50 days and the skin expression of growth hormone receptor (GHR), insulin-like growth factor1 (IGF-1), insulin-like growth factor receptor (IGF- R), KAP3.2 and KAP6-1 mRNA were measured by RT-PCR. The results indicated that IGF-1 could degrade GHR gene expression, have no effect of IGF-1 and IGF-1R gene expression, and increase significantly KAP3.2 and KAP6-1 gene expression. Taken together these findings that the improvement of KAP3.2 and KAP6-1 gene expression may correlate with other pathways beyond the insulin-like growth factor axis.

Keywords: Sheep; gene expression; growth hormone receptor (GHR); insulin-like growth factor1 (IGF-1);insulin-like growth factor receptor (IGF-1R); keratin intermediate filament-associated protein 3.2 (KAP3.2); keratin-associated protein 6-1(KAP6-1)

African Journal of Livestock Extension Vol. 5 2007: pp. 37-42