Addition of anti-Toxoplasma gondii membrane immunoglobulin Y to reduce necrotic index in mice’s liver

Abstract

Background: The study aimes to determine the effect of administering anti-T. gondii membrane IgY against liver damage (Necrotic index) and the effectiveness of the antibody’s delivery time.

Materials and Methods: This research was a laboratory experiment with five treatments and five replicantions. Each treatment used female mice (Mus musculus) as animal models. The treatment groups consisted of a P0 group (not infected), P1 group (infected), P2 group (anti- T. gondii membrane IgY given one day before infection), P3 group (anti-T. gondii membrane IgY given together with infection) and P4 group (anti-T. gondii membrane IgY given two days after the infection. A dose of anti- T. gondii membrane IgY as many as 75 ug/head and infectious dose of 10 tachyzoites/head were given. Four days after infection mice were sacrified and examined. Finally, necrotic index in histopathological liver using Hematoxylin Eosin.

Results: The percentage of necrotic index liver showed that result treatment of P2 and P3 treatment that lower than another treatment.

Conclusion: Thus, it can be concluded that administration of anti-T. gondii membrane IgY can reduce liver cell necrotic index and it was greatest when given before and simultaneously with infection.

Keywords: Toxoplasma gondii, immunoglobulin Y, liver damage