Ecofriendly application of cellulase and xylanase producing marine Streptomyces clavuligerus as enhancer in biogas production from waste

Abstract

The marine actinomycete strain MAC 9 was used for the production of cellulase and xylanase from wheat bran with inducers oats and soy meal as substrate. Out of 30 actinomycetes screened from sediments of Tiruchendhur coastal areas of Tamil Nadu, India only 8 strains showed both cellulase and xylanase activity. The marine actinomycete exhibited highest enzyme activity at alkaline pH 8-9 with temperature ranging from 40-55°C. The enzymes were used in pretreatment of rice and wheat straw waste for biogas  production. More biogas production was observed in the agricultural waste with the pretreatment of  cellulase, xylanase and the combination of enzymes.

Key words: Biogas, cellulase, energy, marine actinomycete, solid state fermentation, thermophilic, wheat bran, xylanase.