Molecular detection of vector-borne bacterial pathogens in dromedary camels from Algeria

Abstract

Background: In Algeria, little focus was placed on camels as hosts of tick-borne bacterial diseases. Recent studies showed a high prevalence of tick infestation in dromedaries. Transmission of various pathogenic micro organisms to camels by ticks imposes considerable economic losses to livestock and greatly impact on human and animal health. The aim of our study was to investigate the occurrence of vector-borne zoonotic bacteria in camels from Algeria.

Methodology: Blood samples were collected from 80 randomly selected camels in Laghouat province, southern Algeria. The samples were screened for Anaplasma spp, Bartonella spp, Rickettsia spp and Coxiella burnetii by qPCR. All positive samples were confirmed by standard PCR followed by sequencing. Data on age, sex, tick infestation and location of the camels were analyzed using the SPSS version 17.0 and association of these with vector-borne bacterial pathogens was determined using Chi-square (χ2) test. P value lower than 0.05 wasconsidered as indicative of significance.

Results: Twenty five of the 80 (31.3%) camels were positive to at least one vector-borne bacterial pathogen with Anaplasma phagocytophilum (22.5%, 18/80) being the most prevalent species, followed by Anaplasma platys (7.5%, 6/80) and Bartonella dromedarii (2.5%, 2/80). Only one camel was co-infected with two pathogens. All samples tested negative for Rickettsia spp and Coxiella burnetii. None of the factors (age, sex, tick infestation and study sites) was significantly associated with prevalence of vector-borne bacteria in the camels (p>0.05).

Conclusion: The present study is the first report of anaplasmosis and bartonellosis in "Camelus dromedaries" from Algeria. Our results highlighted the need for further investigations on tickborne pathogens of camels.