Occurrence of Extended-Spectrum Beta-Lactamase Producing Enterobacteriaceae Isolates in Communal Water Sources in Ogun State, Nigeria

Abstract

The role of Enterobacteriaceae in dissemination and reservoir of antibiotic resistance genes in outbreaks of disease and infections are pressing public health concern. This study is aimed at investigating the antibiotic resistance patterns and ESBL production in water-borne Enterobacteriaceae recovered from some selected drinking water sources among 6 towns in
Ogun State (Nigeria). Sixty water samples were collected from boreholes and well waters sources, of these samples 68 Enterobacteriaceae including Enterobacter spp, Escherichia coli, Klebsiella spp, Salmonella spp, Citrobacter freundii, Serratia spp were recovered and identified presumptively using standard microbiological and biochemical methods. The susceptibilities of the isolates to nine antibiotics were carried out by disk diffusion method and determination of ESBL production was by double-disk synergy method. Of the 68 isolated strains tested, 1 (1.47%) was susceptible to all the
antibiotics, 98.5% of the isolates were resistant to . 1 antibiotics and 75% were found to be resistant to . 2 classes of antibiotics. Incidence of water-borne ESBL-producing enteric in this study was 7.14%. The increasing unabated spread of Enterobacteriaceae in public water supply harboring resistance genes portends a high risk for communal outbreaks. This necessitates an urgent precautionary and antibiotics surveillance measures in Nigeria.

Keywords: Enterobacteriaceae, antibiotic resistance, ESBL