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Thermostability of reconstituted newcastle disease virus strains at 36#&176C temperature
Abstract
Haemagglutination (HA) test was employed to determine the stability of HA titers of reconstituted form of Hitchner – B1 (B1), LaSota (L) and Komarov (K) strains of Newcastle Disease Vaccine (NDV) at 360c. The
temperature treatment method was through incubation (in water bath) of the reconstituted vaccines at selected temperature and sequential sampling of each vaccine vial for the determination of pre – and post – temperature exposure HA titers. Thus, on the basis of a two-step (2log2) decline in titer as evidence of loss of stability of HA titers (LST), the LST therefore, occurred at 50th, 24th and 95th hour for BI, L and K strains, respectively, post –temperature exposure. The data, therefore, showed that the NDV – K strain was the most stable at the test temperature. It is believed that the findings will enhance the understanding of the potential of this strain in the
developed and application of a thermostable MDV target on village poultry. In rural settings.
temperature treatment method was through incubation (in water bath) of the reconstituted vaccines at selected temperature and sequential sampling of each vaccine vial for the determination of pre – and post – temperature exposure HA titers. Thus, on the basis of a two-step (2log2) decline in titer as evidence of loss of stability of HA titers (LST), the LST therefore, occurred at 50th, 24th and 95th hour for BI, L and K strains, respectively, post –temperature exposure. The data, therefore, showed that the NDV – K strain was the most stable at the test temperature. It is believed that the findings will enhance the understanding of the potential of this strain in the
developed and application of a thermostable MDV target on village poultry. In rural settings.
