Bbioautographic evaluation of antibacterial metabolite production by wild mushrooms

Abstract

Russula sp. and Pycnoporus cinnabarinus were subjected to liquid and
solid state fermentation for metabolite production. Local rice substrate
was used for the solid state fermentation to provide a cheap and readily
available medium for laboratory cultivation of wild mushroom.
Bioautography, a technique that combines chromatography with bioassay
in situ allows the localization of the active constituent. The result showed
that in submerged fermentation, both the culture filterate and mycelial
extract of Russula sp. displayed antimicrobial activity while only the
culture filtrate demonstrated activity in Pycnoporus cinnabarinus.. The
activity of the solid state fermentation was exhibited by the ethyl acetate
fractions while the aqueous fraction showed no activity. The comparison
of the culture filtrates (ethyl acetate fractions) of submerged fermentation
and the ethyl acetate fractions of solid state fermentation displayed
similar activities (Afr. J. Biomed. Res. 9: 57 – 62, 2006)

Keywords: Bioautography, Mushroom, Metabolite, Solid state, Fermentation.