Evaluation of antibacterial activity and phytochemical analysis of Bauhinia variegata l. bark

Abstract

Bauhinia variegata L. bark powder was defatted with petroleum ether.
The non-defatted plant material as well as the defatted plant material was
then individually extracted in different solvents with increasing polarity
viz. 1,4-dioxan, acetone, methanol, dimethylformamide (DMF) and
distilled water respectively. The extractive value of B. variegata L. for
non-defatted extracts ranged from (0.7-13%) and for defatted extracts the
extractive value ranged from (1-10.5%). The antibacterial activity of all
the extracts (non-defatted and defatted) of Bauhinia variegata L. bark
was determined by agar well diffusion method at three different
concentrations i.e., 10 mg/ml, 5 mg/ml and 2.5 mg/ml. The antibacterial
activity of defatted extracts of Bauhinia variegata L. was more than those
without defatting. Maximum activity was observed at highest
concentration i.e. 10mg/ml. Defatted acetone and methanol extracts of
Bauhinia variegata L. were most active as compared to other extracts
against all the studied organisms. Petroleum ether extracts of Bauhinia
variegata L. was inactive against all microorganism (Afr. J. Biomed. Res.
9: 53 – 56, 2006)

Keywords: Antibacterial activity, Bauhinia variegata L. non-deffated extracts, defatted extracts.