In Vitro evaluation of apoptotic effects of different Viruddha samyogas of kshira in human hepatic WRL-68 cell line

Abstract

Background: Viruddhahara, the incompatible food combinations explained in Ayurveda has deleterious effects on the body even affecting the progeny. Detailed understanding of the cellular level impact of viruddhahara is imperative. In this study the apoptotic effects of incompatible combinations of kshira were evaluated. Materials and methods: The apoptotic effect of viruddha kshira samyogas with lavana, kadali, maasha, matsya and dadima was studied on the human hepatic WRL-68 cell line using fluorescent assay with Acridine orange/Propidium Iodide dual staining method. The morphological changes were observed in all the groups. Results: All the viruddha combinations of kshira exhibited apoptotic changes of varying degrees. Early apoptotic changes like nuclear fragmentation, chromatin condensation, blebbing of cell membrane and late apoptotic changes and secondary necrotic cells were the morphological changes observed in the groups. Cells treated with kshira alone showed limited late apoptosis changes while 90% of the cells were viable. Discussion & Conclusion: Viruddha kshira samyogas have apoptotic effects on WRL-68 cell line. From this study, it is known that viruddhahara sevana is detrimental to health. Eating habits and lifestyle plays vital role in causation of diseases. Hence, choosing diet as suggested in Ayurveda texts is a promising target for lifestyle interventions.