Effect of unripe Musa paradisiaca fruit diet on iron-induced renal impairment in rats

Abstract

Unripe cvc fruit meal has been suggested to possess antioxidant potentials. This study thus investigated the effect of unripe Musa paradisiaca fruit (UMP) diet on iron-induced renal impairment, a likely consequence of iron-induced oxidative stress, in Wistar rats. Thirty-rats were equally divided into five groups. Group 1 (control) received standard rat chow only. Renal impairment via oxidative stress was induced with ferrous-sulphate (3mg/kg, i.p.) in groups 2–5 and animals were simultaneously maintained on standard rat chow (group2), 20%UMP-diet (group3), 40%UMP-diet (group4) and 80%UMP-diet (group5) respectively, for 28days. Thereafter blood samples were obtained from the retro-orbital sinus after light di-ethyl ether anesthesia into plain sample bottles. Serum was obtained from these samples and analyzed for urea and creatinine levels. Kidney samples were also obtained from each animal for histological evaluation using H and E stains. Urea and creatinine were significantly reduced in groups 4 and 5 compared to group 2. Animals in group 2 (iron only) had kidneys samples with poor architecture; renal cortices had moderate peri-glomerular, glomeruli and perivascular infiltration. The renal tubules in this group also lacked luminar space and exhibited epithelia depletion. These pathologies were partially prevented in groups 3 and 4 while group 5 (80%UMP-diet) showed kidney samples that were comparable with control. This study suggests that unripe Musa paradisiaca rich diet may reduce iron-induced renal impairment.

Keywords: Unripe Musa paradisiaca , renal function, renal impairment, iron

Afr. J. Biomed. Res. Vol. 22 (January, 2019); 101- 104