Gastroprotective Properties of Manganese Chloride on Acetic Acid Induced Ulcer in Wistar Rats

Abstract

Drugs with multiple mechanisms of protective action may be effective in minimizing tissue injury during diseases. Manganese has shown varied positive biological properties in reverting diseased conditions. There is dearth of information regarding its effects on gastrointestinal integrity, hence the aim of this study. Seventy male Wistar rats used in this study were randomly assigned into 7 groups viz: Groups 1 – control, 2 – ulcer untreated rats, 3,4,5,6 and 7 received 50mg/kg, 100mg/kg Manganese, 40mg/kg Cimetidine, 1mg/kg Mistoprotol and 30mg/kg Omeprazol respectively. Two weeks oral treatment (in groups 3-7) began 3 days after laparotomy of which chronic gastric ulcer was induced using acetic acid. On days 7 and 14, gastric acid secretion was performed on animals (fasted for 24 hours) from each group and their stomachs were removed, weighed and scored for ulcer before histological evaluation. Data were analyzed using ANOVA & Student t-test and significant at p<0.05. Results revealed that Manganese had dose and treatment duration dependent effect on healing of ulcerated stomach. Ulcer index in Manganese (50 and 100mg/kg) treated groups significantly reduced on days 7 and 14 (77.5% and 94.1%) and (93.8% and 100%) respectively compared with ulcer untreated group. Anti-secretory property was more prominent in animals treated with Omeprazole while Cimetidine treated animals showed similar trends with Manganese (100mg/kg). These results were further buttressed by histological findings that revealed no ulcer in Manganese treated groups. Findings of this study suggests that manganese might possess gastro-protective and anti-secretory activities.

Key words: Manganese Chloride, Acetic acid, Ulcer, Rat