β-Cyclodextrin-curcumin complex inhibit telomerase gene expression in T47-D breast cancer cell line

Abstract

Today, attempt to the preparation of stable drug with high drug delivery efficiency is inevitable. Curcumin (diferuloylmethane), with hydrophobic structure obtained from the herb of Curcuma longa, have various applications in cancer therapy. But, its low water solubility and bioavailability is possible for poor drug delivery of curcumin. In this study, we prepared β-cyclodextrin-curcumin complex to determine the inhibitory effect of this drug on telomerase gene expression. Curcumin was encapsulated into cyclodextrin and the rate of curcumin loading was estimated. Cytotoxic effects of β-cyclodextrin curcumin were investigated by colorimetric cell viability (MTT) assay. Then inhibition of telomerase gene expression was determined by real-time polymerase chain reaction (PCR). MTT assay demonstrated that β-cyclodextrin have no cytotoxic effect on its own. Also, it showed dose-dependency and time-dependency for β-cyclodextrin–curcumin on T47D cell line. Expression of telomerase gene in cells effectively was reduced as the concentration of β-cyclodextrin –curcumin complex was increased. The results show that β-cyclodextrin -curcumin complex have cytotoxic effect on T47D cell line through down regulation of telomerase expression and induction apoptosis by enhancing curcumin uptake by cells. So, β-cyclodextrin could be good carrier for these kinds of hydrophobic agents.

Key words: Anti cancer drug, target therapy, telomerase, breast cancer, drug delivery