Direct organogenesis of Aegle marmelos (L.) Corr. from cotyledon explants

Abstract

Cotyledon segments from in vitro seedlings of Aegle marmelos were cultured on Murashige and Skoog (MS) medium supplemented with different concentration and combination of growth regulators. Highest regenerative response was observed in the medium containing 1.5 mg/l BA + 0.2 mg/l IAA in which 80% of the explant produced shootlets. After 2 sub-cultures in the same media composition, the shoot bud clusters that developed from cotyledon segments had started elongation of shootlets to a reasonable length of 3 - 5 cm. Elongated shoots could be rooted onto half-strength MS medium supplemented with 4 mg/l IBA with 0.2% activated charcoal.

Key words: Cotyledon segments, growth regulator, shoot bud induction.