A novel filarial topoisomerase II inhibitor produced by native isolate Micrococcus luteus B1252

Abstract

A novel inhibitor of filarial topoisomerase II was isolated from the culture filtrate of native isolate Micrococcus luteus B1252. The methanolic extract of the cell pellet was partially purified by silica column chromatography and preparative high performance liquid chromatography (HPLC). The active fraction obtained from HPLC was subjected to gas chromatography-mass spectroscopy (GC-MS) analysis which revealed the presence of 11 compounds. The fatty acid ester derivatives (Z) 15- tetracosenoic acid, methyl ester present in the partially purified extract of M. luteus B1252 is suggested to be the active principle which inhibits topo II.

Key words: Filariasis, topoisomerase II, Micrococcus luteus B1252, fatty acids, (Z) 15-tetracosenoic acid, methyl ester.