Analysis of the early development in first and backcross generations between Paralichthys olivaceus and Paralichthys dentatus

Abstract

This study investigated the performance difference of reciprocal hybrids and backcrosses between Paralichthys olivaceus and Paralichthys dentatus. The fertilization and hatching rates, combined fitness measure, early developmental characteristics and chromosome number were analyzed. The crosses of P. olivaceus ♀ × P. dentatus ♂ (F1), F1♀ × P. olivaceus ♂ and F1♀ × P. dentatus ♂ could normally fertilize (the fertilization rate: 93.2 to 97.7%), hatch (the hatching rate: 84.4 to 93.1%) and develop during prelarvae stage. However, the fertilization rate (41.7%) and combined fitness measure (0.327) of P. dentatus ♀ × P. olivaceus ♂ was significantly reduced compared with other crosses. And the embryos of P. dentatus ♀ × P. olivaceus ♂ hatched out with flexural spine and poor vitality and died within 3 days post hatching. In addition, the result of cytogenetics analysis showed two chromosomes were missing in P. dentatus ♀ × P. olivaceus ♂, which provided evidence of postzygotic barriers in the two species. The results would be useful for better understanding the genetic phenomena of the distant hybridization in fish species.

Key words: Paralichthys olivaceus, Paralichthys dentatus, early development, inter-specific hybridization,