Conditions for regeneration and transformation of Solanum tuberosum cultivars using the cotton glutathione S-transferase (Gh-5) gene

Abstract

Our aim was to produce multiple stress-resistant plants containing the glutathione-S-transferase (Gh-5) auxin-regulated gene, which is related to the presence of the stress resistance protein and is expressed in the transgenic plant genome. We used the Agrobacterium-mediated transformation method. In all tested cultivars (Chubaek, Jasim, Jopoong, Namjak and Sumi), there were higher regeneration frequencies from internode explants than from leaf explants. Incorporation of the GST gene into potato was confirmed by PCR analysis of genomic DNA that demonstrated the 585 bp band. Southern blot analysis confirmed the integration of the Gh-5 gene into the genomic DNA of both forms. No such bands were obtained from non-transformed potato DNA; double bands were detected in digested DNA from transformed potato plants.

Key words: Glutathione-S-transferase gene (Gh-5), potato, transformation, transgenic plant.