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Comparative proteomic analysis of the effects of dietary mugwort (Artemisia iwayomogi Kitamura) on the pig Longissimus dorsi muscle


BU Kim
MA Jeong
YS Ryu
HC Park
JH Jung
SW Kim
CW Kim
YM Song
KH Chung
IS Kim
SK Jin
SS Lee
IS Choi
KK Cho

Abstract

Proteomic analysis by 2-dimensional electrophoresis (2-DE) of the longissimus dorsi muscle in finishing pigs (LY × D) grown on a diet supplemented with mugwort powder found roughly 300 spots on a polyacrylamide gel image. Among them, the expression levels of 12 spots were higher than those of the control group. From the results of peptide mass fingerprinting (PMF) analysis, nine proteins were characterized: myosin light chain 2V, elF-5A, myosin light chain 1, F1-ATPase chain D, peroxiredoxin-2, Kelch-related protein 1, SLA-7, glycine amidinotransferase, and Tpi 1. The other three spots with increased expression were identified by chemically assisted fragmentation-matrix-assisted laser desorption/ionization (CAF-MALDI) sequencing as serum albumin precursor and two different types of myoglobin. Taken together, these results indicate that mugwort powder has the potential to improve the quality of pork meat.

Key words: 2-DE, longissimus dorsi muscle, meat quality, mugwort, pig, proteome.


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eISSN: 1684-5315