Mutation analysis of the negative regulator cyclin G2 in gastric cancer

Abstract

Cyclin G2 is an unconventional cyclin which might have a potential negative role in carcinogenesis. In this study, the effect of cyclin G2 overexpression on gastric cell proliferation and expression levels of cyclin G2 in normal gastric cells and gastric cancer cells were investigated. Moreover, mutation analysis was performed in tissues from 57 gastric cancer patients. Our data demonstrate that ectopic expression of cyclin G2 inhibited the proliferation of gastric cells and the expression of cyclin G2 were significantly decreased in gastric cancer cells compared with normal cells. No mutations were found but four synonymous SNPs were detected in gastric cancer tissues, suggesting that mutations of cyclin G2 might not be the main reason for gastric carcinogenesis.

Key words: Cyclin G2, gastric cancer, negative regulator, mutation screen.