Non liquid nitrogen-based-method for isolation of DNA from filamentous fungi

Abstract

A simple, efficient, reliable and cost-effective method for isolation of total genomic DNA from fungi, suitable for polymerase chain reaction (PCR) amplification and other molecular applications was described. The main advantages of the method are: (1) does not require the use of liquid nitrogen for preparation of fungi DNA; (2) the mycelium is directly recovered from Petri-dish cultures; (3) the quality and quantity of DNA obtained are suitable for molecular assays; (4) the technique is rapid and relatively easy to perform; (5) it can be applied to filamentous fungi from soil as well as from a fungi from other environmental sources; and (6) it does not require the use of expensive and specialized equipment or hazardous reagents. This method does not require liquid nitrogen for fixation, grinding or storage at - 80°C, making it advantageous over other common protocols.

Key words: Genomic DNA extraction, filamentous fungi, polymerase chain reaction (PCR) amplification.