Cloning and sequence analysis of the defective in anther dehiscence1 (DAD1) gene fragment of Chinese kale

Abstract

To clone the defective in anther dehiscence1 (DAD1) gene fragment of Chinese kale, about 700 bp product was obtained by PCR amplification using Chinese kale genomic DNA as the template and a pair of specific primers designed according to the conserved sequence of DAD1 genes of Arabidopsis thaliana and Brassica rapa. The amplified product was ligated into the T vector and sequenced. The results show that the gene fragment was 678 bp long without introns. It shared 89% identity with the nucleotide sequence of the DAD1 gene of A. thaliana and the sequence identity was as high as 97 to 99% with those of other plants belonging to the same genus as Chinese kale. The amino acid sequence deduced from the nucleotide sequence had 91% identity with that of A. thaliana. It was shown that the cloned fragment was a part of Chinese kale DAD1 gene.

Key words: Chinese kale, Brassica oleracea var. alboglabra, defective in anther dehiscence1 (DAD1), gene clone.