Endothelial nitric oxide synthase gene Glu298Asp polymorphism and risk of preeclampsia in South East of Iran

Abstract

Preeclampsia (PE) is the most serious complication of pregnancy that causes maternal and fetal morbidity and mortality. Although the exact pathophysiology of PE is unknown, a large number of studies have shown that abnormalities in nitric oxide (NO) synthesis may contribute to the development of this disorder. There are some evidences that polymorphisms of the endothelial nitric oxide synthase (eNOS) gene affect NO production and have been associated with hypertension and PE in some populations. Therefore the aim of this study was to assess the relation of the Glu298Asp eNOS polymorphism and PE in an Iranian population. We compared the frequency of the Glu298Asp polymorphism in 147 women with PE and 137 healthy pregnant control subjects by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method. The frequencies of Glu298Asp genotypes were significantly different between PE women and controls (p < 0.001). The frequency of Asp allele was 0.32 in PE patients and 0.20 in controls and was significantly different (p < 0.001). The risk of PE was 2.4 fold in pregnant women with Asp allele. In conclusion, the Asp allele could be a risk factor for PE in South East of Iran.

Key words: Nitric oxide synthase, polymorphism, preeclampsia, pregnancy.