Molecular cloning and sequence analysis of the cat myostatin gene 5´ regulatory region

Abstract

Cat is an important experimental and pet animal and plays a key role in keeping ecological balance. Myostatin is a negative regulator of skeletal muscle growth and development in mammals, but the gene sequence of it keeps unknown in cat. To better understand the structure and function of the myostatin promoter in cat, a 1409 bp fragment containing the 5'-regulatory region of the cat myostatin gene was cloned and sequenced (GenBank accession number is GU938462). Many potential transcription factor binding sites have been found by the bioinformatics analysis, such as TATA boxes, CAAT box, E-boxes, MEF2, MEF3, MTBF, PAX3, SMAD, HBOX, HOMF and TEAF motifs. Comparative analysis for some motifs showed both conservations and differences among cat, horse, porcine and human.

Key words: Cat, myostatin 5'-regulatory region, molecular cloning, sequence analysis and comparison, transcription factor binding sites.