Haematological and immunological effect of coadministration of extracts of Vernonia amygdalina and Azadirachta indica on normal and diabetic rats

Abstract

This study evaluated the effect of co-administration of extracts of Vernonia amygdalina Del. (VA) and Azadirachta indica Linn.(AI) on haemapoietic and immunological indices of normal and diabetic rats. White blood cells which were non-significantly decreased (p>0.05) in diabetic control rats relative to the normal control, respectively increased and decreased non-significantly (p>0.05) upon administration of the combined extracts of VA and AI to diabetic and non-diabetic test rats. Packed cell volume, haemoglobin content and red cell count as well as its derived factors (mean cell volume, mean cell haemoglobin and mean cell haemoglobin concentration) of both diabetic and non-diabetic rats were not affected by the treatment, relative to their respective controls. As compared to insulin treatment, the combined extracts significantly increased (p<0.05) mean cell haemoglobin concentration (MCHC) of diabetic rats. Platelets number which was non-significantly lower (p>0.05) in diabetic control rats as compared to non-diabetic control was further decreased non-significantly (p>0.05) upon administration of the combined extracts and insulin. Diabetes induction significantly increased CD₄⁺ count (p<0.05) as compared to the normal control. This was however decreased significantly (p<0.05) upon treatment with the combined extracts and insulin. The combined extract similarly decreased CD₄⁺ counts in normal test rats as compared to the normal control. Combined extracts of VA and AI is non-haematotoxic and may possess some anti-inflammatory properties when used as a management against diabetes mellitus.

Key words: Diabetes, haematological and immunological indices, Vernonia amygdalina, Azadirachta indica.