Enhancement of callus induction and regeneration efficiency from embryo cultures of Datura stramonium by adjusting carbon sources and concentrations

Abstract

Carbon source in the medium is considered to be an essential component for the high production costs of callus and plantlets in tissue culture. We report here the establishment of an efficient tissue culture cycle (callus induction and plant regeneration) for Datura stramonium by adjusting carbon sources and concentrations. Embryo explants of D. stramonium L. were cultured in vitro with six different carbon sources (sucrose, glucose, fructose, galactose, maltose and lactose) and four concentrations (1, 2, 3 and 4%) for callus induction. After that, the best carbon sources for regeneration were investigated with four different carbon sources (sucrose, glucose, fructose and sorbitol) and four concentrations (1, 2, 3 and 4%). The best sugar for callus induction was 2% lactose. The medium containing 3% glucose shows the higher regeneration.

Key words: Datura stramonium L., carbon source, callus induction, plant regeneration and embryo culture.