Antibacterial activity of various fractions of ethyl acetate extract from the desert truffle, Tirmania pinoyi, preliminarily analyzed by gas chromatography-mass spectrometry (GC-MS)

Abstract

The antibacterial activity of various fractions of ethyl acetate extract isolated from edible fungi, Tirmania pinoyi (Maire) Malençon, growing in Algeria, was investigated. Extraction was done by the Soxhlet and the fractions obtained were purified with silica-gel column. Two fractions of ethyl acetate extract were tested against the bacteria Escherichia coli ATCC 25922, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 14028 and Enterococcus sp. ATCC 29212 using the agar diffusion technique. These fractions were preliminary analyzed by gas chromatography-mass spectrometry (GC-MS). The results show inhibition zones, with diameters between10 and 22 mm, against B. subtilis ATCC 6633 and Staphylococcus aureus ATCC 6538. The fractions were not effective against the other investigated bacteria. The GC-MS showed the presence of several products, some of them have antibacterial activity in the literature.

Key words: Edible fungi, desert truffle, Tirmania pinoyi, antibacterial activity, gas chromatography-mass spectrometry (GC-MS), ascocarps.