A simple and rapid plate assay for screening of inulindegrading microorganisms using Lugol’s iodine solution

Abstract

In this report, a simple and rapid agar plate assay was established for screening of halophilic, inulindegrading microorganisms. Two strains considered inulinolytic with this method were chosen and the inulinolytic activities in their culture supernatant were measured with the Somogyi-Nelson method, while their hydrolysis products of inulin were detected with TLC chromatogram.

Key words: Screening, halophilic microorganism, inulinase, Lugol’s iodine solution.