Different patterns of transcriptomic response to high temperature between diploid and tetraploid Dioscorea zingiberensis C. H.

Abstract

Polyploidy is an important evolutionary force in plants and may have significant impact on plant breeding. In this study, expression changes between diploid and tetraploid Dioscorea zingiberensis C. H. under control and high temperature conditions were investigated by sequence-related amplified polymorphism (SRAP)-cDNA display approach. Up to 2.7% of the expression changes induced by genome doubling were detected in the tetraploid D. zingiberensis relative to its diploid progenitor. Under high temperature stress, a “random transcriptome response” pattern employed with 6.3% of the expression changes were detected in diploid plants, while, an “activation transcriptome response” pattern developed with 6.9% expression changes were detected in tetraploid plants. This result indicated that there might be ploidy dependent pattern of transcriptomic response to high temperature environment, which might contribute to the evolutionary success of polyploids.

Key words: Dioscorea zingiberensis C. H., high temperature, polyploidy, sequence-related amplified polymorphism -cDNA.