A random amplified polymorphic DNA (RAPD) molecular marker linked to late-bolting gene in pak-choi (Brassica campestris ssp. chinensis Makino L.)

Abstract

Bulked segregant analysis (BSA) and random amplified polymorphic DNA (RAPD) methods were used to analyze F₂ individuals of P-27 × P-28 to screen and characterize the molecular marker linked to latebolting gene in pak-choi (Brassica campestris ssp. chinensis Makino L.). A total of 200 random primers were used for RAPD analysis. One RAPD marker S265₇₅₀ was identified to be co-segregating with the late-bolting gene, and the genetic distances between S265₇₅₀ and late-bolting gene was 3.14 cM. The results of the study can be seen as a starting point for future researches on the pak-choi bolting gene mapping and molecular marker assisted breeding.

Key words: Pak-choi, late bolting, random amplified polymorphic DNA (RAPD), bulked segregant analysis (BSA).