Effects of several physiochemical factors on cell growth and gallic acid accumulation of Acer ginnala Maxim cell suspension culture

Abstract

The production of gallic acid in cell suspension culture of Acer ginnala Maxim was studied. Some physiochemical factors and chemical substances effect on the cell growth and the production of gallic acid were investigated. Cells harvested from plant tissue culture were extracted and applied to high performance liquid chromatography to measure gallic acid content. 0.008 mg.L^-1 TDZ and 0.1 mg.L^-1 BA was optimal for the cell growth. 0.004 mg.L^-1 TDZ and 0.1 mg.L^-1 BA was best for the production of gallic acid. Maintaining the initial pH value at 5.8 was most suitable for gallic acid accumulation in A. ginnala Maxim cell suspension cultures. To satisfy the condition of mass-producing gallic acid in the suspension culture, the adapted inoculum quantity was 30 g.L^-1. The results also provided evidence that the optional culture period was 7 days with light.

Key words: Acer ginnala Maxim, gallic acid, cell growth, suspension culture, physiochemical factor.