Inheritance of DNA methylation in DH and its backcrossed lines of Brassica napus

Abstract

Nineteen (19) pairs of methylation sensitive amplified polymorphism (MSAP) primers were used for analysing DNA methylation in two DH lines and their two back-crossed generations, namely BC1 and BC2. Constitutive DNA methylation was stable across three generations and inherited changes in it were of six types. 60 fragments were chosen for sequencing and then, were analysed using the Blast database and the strength of their expression was assessed. Two types of genes, those related to retrotransposons and those involved in other functions, were identified but the degree of expression of
those genes was lower in the progeny than in the parents.

Key words: DNA methylation, methylation sensitive amplified polymorphism (MSAP), backcross line, Brassica napus.