Studies on Buddleja asiatica antibacterial, antifungal, antispasmodic and Ca++ antagonist activities

Abstract

Crude extract of Buddleja asiatica Lour and its fractions, chloroform (F1), ethyl acetate (F2) and nbutanol (F3) were evaluated for antibacterial, antifungal, antispasmodic and Ca⁺⁺ antagonist activities. The antibacterial activity was performed against 11 types of bacteria. The crude extract and fractions F2 and F3 exhibited significant activity, while F1 showed low activity in killing the Shigella flexenari, Sternostoma boydi and Escherichia coli. In the rest bacteria, the crude extract and all the fractions (F1 to F3) revealed minimum to nil inhibitory effect. The fungicidal activity of the crude extract and all the fractions (F1 to F3) was also performed against six different fungi. The crude extract and fractions F1 and F3 displayed significant activity, while fraction F2 showed moderate activity against Fusarium solani. In the case of Microsporum canis, the crude extract and fraction F3 showed high activity but in the other four fungi, the inhibition area exhibited optimum to nil activity in crude extract and all the fractions (F1 to F3). In isolated rabbit jejunum preparations, B. asiatica crude extract caused concentration-dependent (0.03 to 1.0 mg/ml) relaxation of spontaneous and high K⁺ (80 mM)-induced contractions. The results indicate the antibacterial, antifungal, antispasmodic and Ca⁺⁺ antagonist potential of B. asiatica Lour.

Key words: Buddleja asiatica, antibacterial, antifungal, antispasmodic, Ca⁺⁺ channel blocker.