Inhibition of bacterial adhesion to HT-29 cells by lipoteichoic acid extracted from Clostridium butyricum

Abstract

The aim of this experiment was to study the effect of the lipoteichoic acid (LTA) extracted from Clostridium butyricum on the adhesion of C. butyricum and Escherichia coli to HT-29 human intestinal cells. The method of extraction of lipoteichoic acid form C. butyricum by TX114 was evaluated. The purification of the LTA by DEAE-cellulose 52 anion exchange chromatography was also investigated. In addition, the LTA was assayed for its inhibition of the adhesion of C. butyricum and E. coli to HT-29 cells and antimicrobial activity. Our results showed that LTA could be extracted by TX114 and purified by DEAE-cellulose 52 anion exchange chromatography and could inhibit the adhesion of C. butyricum and E. coli to HT-29 cells. This result also revealed that the LTA from C. butyricum could inhibit the adhesion of C. butyricum and E. coli to intestinal cells.

Key words: Lipoteichoic acid, Clostridium butyricum, HT-29 cells, adhesion, Escherichia coli