Measurement of genetic parameters within and between breeder flocks of Arian broiler lines using randomly amplified polymorphic DNA (RAPD) markers

Abstract

The present study was carried out in an attempt to detect the genetic variation within and between four populations of commercial broiler lines using RAPD markers. Thirteen out of twenty random markers which were amplified and generating stable and reproducible bands were selected for genotyping of birds in each broiler lines. The average number of diagnostic bands per primer was 8.08 with an average number of 6.96 polymorphic bands across all breed groups. The highest (94%) and the lowest (73.5%) percentage of polymorphic bands were found within sampled birds of Arian C and D lines, respectively. Among the studied broiler lines, the highest genetic uniformity was found in line A, while the greatest within population genetic diversity was found in line C. Estimation of polymorphic loci, Nei’s gene diversity and intra-population uniformity indices suggested that genetic diversities within Arian commercial lines is high. The four strains of broiler lines clustered into two main groups using UPGMA procedure. In the first main group, the closest genetic distance was found between A and B strains, which were first clustered together and then with birds of C strain. The second main group includes birds from D strain. The mean coefficient of gene differentiation (G_st = 0.368) value reflected a high level of population differences. In total, sixty three RAPD bands were found to be strain dependent specific. Therefore, in order to design new diagnostic primers more effective in genetic discrimination among studied lines, unique bands should be cloned and sequenced.

Key words: Genetic variation, broiler lines, polymorphism, random amplified polymorphic DNA markers.