Immobilization of urease on copper chelated EC-Tri beads and reversible adsorption

Abstract

In the present study, Eupergit C^® macroporous beads were functionalized with amino triazole and characterized by FTIR-ATR and SEM. Cu2+ ions were chelated on the triazole modified Eupergit C^® (EC^®), and then the metal chelated beads were used in the adsorption of urease. Maximum reaction rate (Vmax) and Michaelis-Menten constant (km) were determined for the free and immobilized enzymes. Various characteristics of immobilized urease such as the temperature activity curve, thermal stability, operational stability and storage stability were evaluated. The results demonstrated that triazole functionalized Eupergit C^® beads can be applied to metal sorption and enzyme immobilization.

Key words: Urease, immobilization, Eupergit C^®, triazole, chelating beads.