Detection of species diversity of arbuscular mycorrhizal fungi (AMF) classic and molecular methods from the melon (Cucumis melo L.) plants in Van and its districts

Abstract

Arbuscular-mycorhizal fungi (AMF) from melon plants grown in Van province, were studied by nested-PCR method to establish colonization ratio of related fungi in plants and to detect the fungi at species level. From 10 different locations, a total of 100 soil samples were taken from rhizosphere area of melon plants. It was also determined that 52% of these plants were colonized by arbuscular-mycorhizal fungi and were able to establish symbiotic relationship in variable ranges (4.5 to 37.8%) Glomus intraradices and Glomus mosseae were two identified AMF.

Key words: Arbuscular-mycorhizal fungi (AMF), melon, Glomus intraradices, Glomus mosseae, Van.