Optimization of somatic embryogenesis induction in Iranian melon (Cucumis melo cv. Khatooni)

Abstract

In this study, regeneration of Cucumis melo cv. Khatooni: one specific Iranian melon- via somatic embryogenesis was investigated. Three different types of explant (cotyledon, hypocotyl and cotyledonary petiole) from six days old seedlings were used. Growth regulator treatments were two levels of 6-benzyl aminopurine (0 and 0.1 mg/l) and four levels of 2,4-dichlorophenoxyacetic acid (0, 2.5, 5 and 7.5 mg/l). After seven weeks, cotyledonary petiole showed the highest potential in somatic embryo induction and the combination of 0.1 mg/l BA and 5 mg/l 2,4-D had significant effect on somatic embryogenesis of Khatooni melon.

Key words: Melon, somatic embryogenesis, regeneration, cotyledon, hypocotyl, cotyledonary petiole.