Amplified fragment length polymorphism (AFLP) studies on Indian Cycas species

Abstract

Amplified fragment length polymorphism (AFLP) technology was used to reveal the genetic variation in six species of Cycas collected from eleven natural populations. Two sets of primer with 4-selective nucleotides were used in this study and 78% polymorphism was found. The results correlated with geographical distribution of the plants and grouped into four. This study is highly useful in the delimitation of Indian Cycas species which has been enunciated as highly endangered taxa by IUCN Red Data List.

Key words: Cycads, Cycas, amplified fragment length polymorphism (AFLP), polymorphism.