Preliminary study on the inducement effect of colchicine during microsporogenesis of Ginkgo biloba L.

Abstract

This is the first report of colchicine treatment effect on microsporogenesis of Ginkgo biloba L, By high performance liquid chromatography (HPLC) analysis, colchicine was detected in microsporocyte inclusion from colchicine-treated microsporangium and microsporocyte of G. biloba, The change amount of colchicine was detected in mixture liquid after the protoplasm of G. biloba was co-cultured with colchicine by different conditions. Fixed with Carnoy's fluid and stained with improved phenol fuchsin, the development of colchicine-treated microsporogenesis could be examined with microscope. The results are as follows: The colchicine could intrude into treated microsporangium and microsporocyte, but intruded amount of colchicine was different when treatment conditions were different; the change of colchicine content in co-cultured mixtures liquid was few; and there were high frequencies of development tardiness in colchicine-induced microsporangium (more than 70%), but only a few dyads and triads were detected in meiotic products (less than 10%). These observations suggest that 2n pollen can be induced by colchicine but treatment conditions and slowing development of colchicine-treated microsporocyte may affect the inducement effect of colchicines.

Key words: Ginkgo biloba L., microsporogenesis, colchicine, 2n pollen, high performance liquid chromatography (HPLC).