An efficient protocol for in vitro propagation of Rosa gruss an teplitz and Rosa centifolia

Abstract

Rose is a beautiful flower having commercial and ornamental value. In order to establish protocol shoot tips explants of Rosa gruss an teplitz and Rosa centifolia were proliferated in vitro using MS medium supplemented with different levels of benzylaminopurine (0, 0.5,1.0, 1.5, 2.0, 2.5 and 3.0 mg l^-1 ). Maximum numbers of shoots (3.906), shoot length (3.106 cm), fresh weight (178.47 mg) and dry weight (43.06 mg) was recorded at 1.0 mg l^-1 BAP. For induction of root, uniform micro-shoots were excised and transferred to the rooting medium (1/2 MS macro, micro elements and vitamins) supplemented with 20 g l^-1 sucrose and different concentrations (0.00, 0.25, 0.50, 1.0, 1.5 and 2.0 mg l^-1) of indole-3-butyric acid (IBA). IBA increased culture rooting percentage (89.375), number of roots (8.7188) and root length (3.5781 cm) more efficiently at 0.50 mg l^-1.

Key words: In vitro propagation, BAP, indole-3-butyric acid (IBA), Rosa gruss an teplitz, Rosa centifolia.