Fluorescent amplified fragment length polymorphism (AFLP) analysis of genetic diversity and relationship of Chinese Rosa rugosa germplasm resources

Abstract

Rosa rugosa is an excellent ornamental plant with important ecological, economical and medicinal values in China. Polymorphism amplifications of the genomic DNA of 5 wild R. rugosa accessions and 25 cultivars that originated from China with fluorescent amplified fragment length polymorphism molecular markers were investigated in this study to evaluate their genetic diversity and relationships. A total of 1771 clearly identifiable bands (60 ~ 500 bp), including 1704 polymorphic bands, were obtained through amplification using 10 pairs of primers. The average percentage of polymorphic locus was 96.2%, which indicated the high genetic diversity of Chinese R. rugosa germplasm resources. Different quantities of specific bands were detected by 10 pairs of primers from 24 accessions, which could be used to identify the specific R. rugosa accession. There was high similarity between Chinese R. rugosa germplasm resources, as indicated by the similarity coefficient of 30 R. rugosa accessions between 0.4977 and 0.8410, with an average of 0.624. Clustering results suggested that wild accessions from different areas of China were remotely related with the ample genetic diversity. Wild accessions and cultivars also had remote genetic relationship. However, the main Chinese R. rugosa cultivars had high similarity, low genetic differences and a narrow genetic background.

Key words: Rosa rugosa, genetic diversity and relationship, amplified fragment length polymorphism.