Isolation, purification and identification of bacteria from the shoes worn by children

Abstract

13 strains of bacteria were isolated from 12 shoes that were worn by children aged 6 to 12 for more than half a year. Through morphological observation, physiological and biochemical measurements, as well as 16SrRNA sequence analysis, the bacteria were identified as follows: Bacillus licheniformis, Bacillus subtilis (5 subspecies), Bacillus spore (3 subspecies), Bacillus anthracis, Staphylococcus aureus, Bacillus amyloliquefaciens and Bacillus thuringiensis. The results may contribute to the selection of efficient antimicrobial agents for children's shoes and insole.

Key words: Children’s shoes, bacteria, isolation, identification.