Detection of genomic instability in hypospadias patients by random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR) method

Abstract

Hypospadias is a urogenital malformation, and it is a common inborn disorder in male individuals. The etiology of hypospadias is still unsolved. The present study is aimed to identify the genetic instability in hypospadias patients. Random amplified polymorphic DNA (RAPD), a polymerase chain reaction (PCR) based technique, was adopted using ten random primers in twelve cases and twelve controls. The primer detectability on genomic instability in 12 samples ranged from 25% with primer OPA-01 to 66% with OPA-08. Case 2 showed the highest genomic instability (80%). The lowest genomic instabamility was (10%) case 6. The results determined numbers of genomic instabilities among hypospadias patients. In addition, the RAPD-PCR technique is a powerful tool for detection of genomic instability in hypospadias patients. Further larger studies are needed, which include low and high grade of patients to: 1) Obtain RAPD markers useful for hypospadias early diagnosis; 2) investigate different genes directly involved in the etiology of hypospadias; 3) analyze chromosomal instability among hypospadia patients.

Key words: Hypospadias, random amplified polymorphic DNA (RAPD), genomic instability.