Diversity of a microsatellite locus in the CSN1S1 gene in goat populations raised in the southeastern region of Turkey

Abstract

The purpose of this study was to investigate the diversity of the microsatellite locus at the CSN1S1 gene in goats raised in southeastern Turkey. DNA was isolated from blood samples collected from goats raised in Kilis (n= 30), Sanliurfa (n= 30) and Siirt (n= 30) provinces of Turkey. The microsatellite locus was amplified using PCR, and fragment lengths of the PCR products were analyzed using a capillary electrophoresis system. Five alleles were found ranging from 206 to 214 bp. Observed and expected heterozygosities varied from 0.567 to 0.667 and 0.497 to 0.721, respectively. Polymorphism information contents and exclusion probabilities varied from 0.462 to 0.668 and 0.289 to 0.466, respectively. The results suggested that, there were five different alleles of the microsatellite locus of the goat CSN1S1 gene and this microsatellite could be used as a marker for linkage or QTL mapping studies of the goat populations studied.

Key words: Goat, microsatellite, polymorphism, diversity, CSN1S1.