Influence of AgNO3 on somatic embryo induction and development in Manchurian ash (Fraxinus mandshurica Rupr.)

Abstract

In this present study, we explored the effects of silver nitrate (AgNO₃) on somatic embryo induction and the development from immature zygotic embryos in Manchurian ash (Fraxinus mandshurica Rupr.). AgNO₃ played a minor role on in vitro embryo induction frequency and in the number of somatic embryos per explant. However, 1 mg L^–1 AgNO₃ enhanced synchronization and significantly inhibited abnormal somatic embryo formation suggesting that AgNO₃ might serve an important function in controlling the development of somatic embryos in Manchurian ash. Our results provided foundation for a future more efficient somatic embryogenesis and regeneration protocol.

Key words: Abnormality, Fraxinus mandshurica, silver nitrate, somatic embryogenesis, synchronization.