Conditions for the differentiation of melanocyte-precursor cells from human cord blood-derived mesenchymal stem cells

Abstract

The loss of skin pigmentation can induce compromised cutaneous immunity, which can result in conditions such as vitiligo. In this study, we evaluated various agents that are able to induce the differentiation of stem cells into melanocytes. We found that a mixture of forskolin (FK), stem cell factor (SCF) and endothelin-3 (EDN-3) induced melanocyte-like morphology in human cord blood-derived mesenchymal stem cells (CB-MSCs). In addition, significant expression of microphthalmia-associated transcription factor-M and tyrosinase-related protein-1 genes was observed. These results suggest that a mixture of FK, SCF and EDN-3 induces the differentiation of melanocyte-precursor cells (MPCs) from CB-MSCs.

Keywords: mesenchymal stem cells, melanocyte-precursor cells, forskolin, microphthalmia-associated transcription factor-M, tyrosinase-related protein-1

African Journal of Biotechnology Vol. 9(36), pp. 5975-5977, 6 September, 2010