A simple, rapid and efficient method of isolating DNA from Chokanan mango (Mangifera indica L.)

Abstract

Total DNA of Chokanan mango (Mangifera indica L.) was extracted from the leaf for the construction of total genomic library. However, the quality of the extracted DNA was often compromised by the presence of secondary metabolites, thus interfering with the analytical applications. Improvement on the quality of the extracted DNA was achieved through the optimization of leaf harvesting stage and modification on the cetyl trimethylammonium bromide (CTAB) DNA extraction procedure. Fully expended, soften and purplish leaf was proved to yield good DNA quality while the addition of polyvinylpyrrolidone (PVP) and β-mercaptoethanol was effective in the removal of secondary metabolites, particularly polyphenolic compounds. The incorporation of high salt washing step was also efficient in removing polysaccharides. This simple, inexpensive and yet reliable method was proved to be successful in yielding sufficient quality and quantity of DNA for the construction of genomic library.

Keywords: Mango, DNA isolation, genomic library, secondary metabolites, polyphenolic compounds

African Journal of Biotechnology Vol. 9(36), pp. 5805-5808, 6 September, 2010