Refined mapping of loss of heterozygosity in Chinese sporadic gastric carcinoma

Abstract

The aim of this study is to explore precise deleted regions where the candidate tumor suppressor genes might be located in Chinese sporadic gastric carcinoma. By searching in Genothon, NCBI and GDB databases, 145 polymorphic microsatellite markers were chosen, at a mean density of approximately one marker every 2 - 4 cM, covering 15 chromosomes. These polymorphic microsatellite markers in gastric carcinoma and adjacent tissue were analyzed via PCR. PCR products were submitted to electrophoresis on an ABI 3730 DNA sequencer. Genemapper3.2 software was used for LOH (Loss of Heterozygosity) scanning and analysis. Comparison between LOH frequency and clinicopathological factors was performed by Fisher’s exact test. 26 refined regions were mapped as candidate regions for TSGs (Tumor suppression genes) in Chinese sporadic gastric cancer. Associations between LOH and clinical information indicated that 6 loci was associated with pTNM stage, 5 with Lauren's type, 4 with lymph nodes metastasis and another 2 with distant metastasis. Through refined deletion mapping, 26 candidate regions, where TSGs may be located, were found and 17 loci were proposed to be used as clinical markers in Chinese sporadic gastric cancer.

Keywords: Gastric carcinoma, refined mapping, loss of heterozygosity (LOH), tumor suppressor genes (TSGs), tumor markers

African Journal of Biotechnology Vol. 9(35), pp. 5754-5761, 30 August, 2010