Insect-resistance and high-yield transgenic tobacco obtained by molecular breeding technology

Abstract

The modified synthesized VHb gene and insectidal gene (GFMcryIA) were transferred to tobacco plants by Agrobacterium-mediated transformation. The bivalent genes were inserted successfully into the tobacco genome and detected by PCR amplification. Southern blot and Western blot analyses showed that VHb gene was expressed in the transgenic plants. Toxicity assay indicated that insectidal gene expressed pesticidal toxin protein. The net weight of transgenic tobacco plants exceeded that of nontransgenic ones by 8%. Compared to non-transgenic tobacco plants, transgenic plants appeared to be high-yielding, insect-resistant varieties.

Key words: VHb gene, insectidal gene, transgenic plant, pest resistance, yield.