Molecular detection of Edwardsiella tarda with gyrB gene isolated from pirarucu, Arapaima gigas which is exhibited in an indoor private commercial aquarium

Abstract

The pirarucu, Arapaima gigas, which was displayed in commercial aquarium was found dead and was submitted for diagnostic examination. Bacteria from different organs of the fish were characterized using Vitek System®2 and showed 98% probability to Edwardsiella tarda. Polymerase chain reaction
(PCR) result showed positive for E. tarda gyrB gene. The 16S rRNA gene was identical and exhibited 99% sequence similarity with the other known isolates of E. tarda available in the GenBank. This paper reports the isolation and detection of E. tarda with the gyrB gene in pirarucu, A. gigas, which was exhibited in an indoor private commercial aquarium in Seoul, South Korea.

Key words: Arapaima gigas, commercial aquarium, Edwardsiella tarda, gyrB gene, pirarucu.