Characterization and identification of Pseudomonas fluorescens NCIM 2100 degraded metabolic products of Crystal Violet

Abstract

Crystal violet (CV), a recalcitrant molecule, used commonly as biological stain and commercial dye, was subjected to biodegradation in this study by Pseudomonas fluorescens NCIM 2100 and its biodegraded metabolic products were identified by UV, 1HNMR and IR Spectrophotometry. During degradation
process CV dye was first broken down into an intermediate compound which was further converted into either 4-benzhydrylidene-cyclohexa-2,5-dienylamine hydrochloride or 4-benzhydrylidene-cyclohexa-2,5-
dienol or 4-benzhydryl-cyclohexa-2,5- diennol in broth medium and were non-toxic in nature. Hence, P. fluorescens can be used for bioremediation of textile effluents containing CV dye in sequential cycles.

Key words: Crystal violet, bioremediation, Pseudomonas fluorescens, metabolic products, spectrophotometry.